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CLINICAL ASPECTS OF LEUKEMIAS (1)

 

DISEASE

AGE/SEX

DISTRIB

CAUSE

SYMPTOMS

ADENOP

TREATMENT

PROGNOSIS

IM

Any; 15-30

Kids are subclinical

M = F

Neg in trop

And blacks

EBV

From saliva or blood transfusion

Flu, fever, sore throat, tired

Onset 3-4 weeks

Cervical

 

heal 6-12 wks or more

Contagious 16 months

CMV

 

 

Bld transfusn

 

 

 

 

PRV

 

 

 

Red skin

 

Donate blood

Can à AML or 

Myeloid metaplasia

Myelofibrosis

 

 

 

 

 

 

Can à AML

Myeloid Metaplasia

Median age

 =  60

 

 

Onset 2 yrs; Fatigue, ↓wt

Night sweats,

kidney stones

 

 

20%à AML

 

Aplastic anemia

 

 

SC, HIM,

Imm supp: Radiation,

Bensol/Benz

Vir: hepatitis

Bleeding,

Infections

 

BMT, chemo

 Can à AML

Leukemia

(in general)

M > F

60% acute: = immature cells

Genetic, virus, triggers: imm, age, chemicals

Acute: Rapid onset and progression.

Relentless cell division

uric acid

 

 

 

ALL (Pre B)

4 years 65% of kids leuk

< Asian/Blk

 

    same

Fatigue, fever, bleeding (nose, gums, cerebral, petic, bruises), freq infect

yes

Aggressive

Chemo combo

Intrathecal inj

Kid w 10 year remission, best prognosis.  Adults w/Ig relapse = bad

ALL (Pre T)

Kids

Adults <50

< Asian/Blk

   same

             same

yes

 

     same

Not as good as B cell

Kids>Adults

AML

Teens-30

Most comm.

Adult leuk

 

   same

             same

 

Aggressive

Chemo combo

 

More severe in older adults

APL

 

Not very

common

   same

             same

 

      same

Poor (grans releaseà DIC), but good Tx

available

AMoL

“Shilling’s”

 

 

  same

         Same, plus Skin/liver Tumors, gum bleeding

 

   Same; BMT

Not good

AMMoL

“Neigle’s”

 

 

Same; CFU-GM Confusion

   Same, especially

 Intracerebral bleeding

 

   Same; BMT

Not good

LABORATORY FINDINGS IN LEUKEMIAS

DISEASE

Diagnostic

RBCs

WBCs

Platelets

Bone Marrow

Organo

megaly

OTHER

IM

Lg #s atypical lymphocytes; monospot +

Anti-EBV

Anemia (rare)

Downey II

(rare)

 

Hepato (10%)

Spleno

(50%)

HAb +

Post-Perfusion Syndrome

CMV

Atypical lymphs;

 

 

 

 

 

Hab neg

Post-Perfusion Syndrome

PRV

Pancytosis

Increased

Increased

Increased

 

 

 

Myelofibrosis

 

 

 

 

Fibrosis

 

Dry tap

Myeloid Metaplasia

 

UnresponAnemia,

Immature,

Abn morph

Increased

10-20,000

Maybe giant platelets;

Bleed/bruise

Hyperplasia

Hepato

Spleno

 

Dry tap

 

Aplastic anemia

Pancytopenia

Decreased

Decreased

Esp PMNs

(Purpura)

Hypoplasia

 

Fetal Hgb

Tissue culture (SC neg, HIM +)

ALL

(Pre B)

more common

Sm lymphblasts

No Auer rods

Scant cytoplsm

No nucleoli

Decreased

Decreased PMNs

Elevated WBC (2/3 cases)

 

Lymphblast take over

Hepato

Spleno

kidney

CNS infiltrate

Mu Chain Stain +

CALLA (CD 10)* (later stage)

TdT +

Sudan neg, MOP neg

ALL

(Pre T)

      same

same

          same

  Same

Same

 

Same

But no CALLA or mu chain

AML

Lg Myeloblasts

Auer rods

Much cytoplsm

1-2 nucleoli

same

15-20,000

some higher or lower

(few: 100,000)

 

Myeloblasts take over

None

Except

testicles

Sudan +

Myeloperoxidase +

TdT neg

PAS neg

APL

promyelocytes

 

 

 

Promyelos

 

 

AMoL

monoblasts

 

No Auer rods

 

Monocytes

 

Sudan neg

MOP neg

AMMoL

Mono/myelo

anemia; blasts, multi-nuc

15-20,000

Auer rods (maybe)

Myelos and Monocytes

Hepato

Spleno

Kidney

 

Hgb decreased

Slight + Sudan and MOP

Muramidase/lysosyme released in plasma/urine

* CALLA (CD10) detected with Mab immunotyping

 

CLINICAL ASPECTS OF LEUKEMIAS (2)

 

 

DISEASE

AGE/SEX

DISTRIB

CAUSE

SYMPTOMS

ADENOP

TREATMENT

PROGNOSIS

Aleukemic

leukemia

 

 

 

 

 

 

 

Leukemia

phoresis

 

CML

Middle age

25-60yrs

M>F

 

Not very common

Radiation, Benzene,

chemicals,

Idiopathic

Onset 2 yrs:

Fatigue, clots, anorexia,

abd fullness,

night sweats,

kidney stones

yes

Gleevec = good

Irradiate spleen

Longer, more predictable course than acute.

Responsive to therapy

Blast crisis = bad

CLL

50-70

M >> F

< Asian/Blk

most common chronic

Idiopathic

Maybe familial

Slow onset

Fatigue, anorexia

 

yes

Chemotherapy: stabilizes for years, but no cure.

Best Px of chronic leuks Abnorm immune à

cancer; Late stage = anemia, clots, PMNs, die from 2° infections

HAIRY CELL

 

 

Same

Uncommon

 

<2% of leukemias

Retrovirus

HTLV-2

Same as CLL

yes

 αINF = good More responsive than CLL, can live years before going downhill

Good, but eventually die from bacterial or fungal infections.

Hodgkin’s Lymphoma

20-50

 

 

Tumors anywhere in lymph system.

Yes

Starts in cervical

Chemo, radiation = good results

Untreated à anemia à death

Multiple

Myeloma

50-60

M = F

 

 

 

Fractures

 

Yes

Chemo, radiation, plasmaphoresis

Can live for a few years, but high proteins in blood à ischemia everywhere.

Burkett’s

Lymphoma

 

African kids

EBV

 

 

 

 

 

 

 

LABORATORY FINDINGS IN LEUKEMIAS

 

DISEASE

Diagnostic

RBCs

WBCs

Platelets

Bone Marrow

Organo

megaly

OTHER

Aleukemic

Smoldering leukemia

anemia

Decreased;

Few leuk cells

PMNs disfunct

Packed with Blasts

 

 

CML

Many cells, all stages of mat

Ph’ chromo

Modest normocyt

50-500,000

lymphs + monos

eos, baso (later)

↑↑↑

and large

Hypercell

Spleno

megaly

LAP <13

uric acid

CLL

Smudge cells = fragile

Slight à progresses to worse

5-10% = hemolytic

Many small abnorm lymphocytes, clefts;

PMNs

all Ig

 

Normal or slight

Same

Liver and spleen

Surface Ig identify them as mature B lymphocytes

 

TRAP neg

HAIRY CELL

 

Hairy shaped

B lymphocytes

 

Same

Same

Same

Same

Same

Looks like CLL

 

Resistance to tartrate (acid phosphatase)

= TRAP +

Hodgkin’s

Lymphoma

Biopsy lumps

Reed Sternberg cells

 

 

 

 

 

 

Multiple

Myeloma

Plasma cells in blood;

Bence Jones proteins in urine

↑ ESR

Rouleaux stacking

 

 

 

 

 

 

Filled up with plasma cells and one type of Ig = monoclonal gammopathy

 

Excess Kappa (light chain)

Atypical lymphocyte = Lg plasma cell

1) Protein electrophoresis

2) Immunoelectrophoresis

3) Immunofixation, (all to id Ig)

 

Burkett’s Lymphoma

 

 

 

 

 

 

Chr translocation: 8 à 2, 14, 22

Oncogene c-myc à enhances growth